Journal: Toxicological sciences : an official journal of the Society of Toxicology
Article Title: Metabolomic analysis reveals metabolic changes caused by bisphenol A in rats.
doi: 10.1093/toxsci/kfu016
Figure Lengend Snippet: FIG. 7. Effects of BPA on expression of Mat1a and Mat2a genes and SAMe in the liver. (A) Effects of BPA on mRNA levels of Mat1a and Mat2a genes in rat liver. Each bar represents the mean ± SEM, n = 6 for each group. The data shown are representative of the results of three independent RT-PCR analysis. Each value was normalized to the mRNA expression of Gapdh, and summary of results is expressed as percentage of control mRNA levels. V, vehicle control group; L, low-dose group (0.5 g/kg/day); H, high-dose group (50 mg/kg/day). Significance level: **p < 0.05 compared with controls. (B) Effects of BPA on the expression of hepatic proteins of MAT1A and MAT2A. Representative western blots show expression of MAT1A (left) and MAT2A (right) and corresponding GAPDH. The lower trace of each panel shows the bar graph summarizing the immunoblot data. Densitometric results are expressed as mean ± SEM (n = 6 for each group). Each value was normalized to GAPDH, and the relative expression levels were generated compared with the control value. Significance level: **p < 0.05 compared with controls. (C) Effects of BPA on hepatic SAMe levels. Results are expressed as mean ± SEM (n = 6 for each group) and percentage of control SAMe levels. Significance level: **p < 0.05 compared with controls.
Article Snippet: The membranes were incubated in blocking buffer for 2 h at room temperature, followed by overnight incubation at 4◦C with rabbit polyclonal antibodies for MAT1A (Proteintech Group Inc, Chicago, IL, 1:2000 dilution) and MAT2A (Beijing Biosynthesis Biotechnology CO., Ltd, Beijing, China, 1:500 dilution), and mouse polyclonal antibodies for GAPDH (Beyotime, China, 1:1000 dilution).
Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Control, Western Blot, Generated
